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Artigo Científico | Restauração | Inglês | 18/10/2004
Autores: G.A. Soares, M. A. S. PINHEIRO, M. G. DINIZ, M.H. FERNANDES
Palavras-chave: human bone marrow cell cultures, osteogenesis, titanium surface treatments
Resumo: Titanium samples of different roughness (Ra) and morphology were prepared using a combination of mechanical with and without chemical attack of hydrofluoric acid based solution treatments. The biological performance of the prepared surfaces was evaluated using human bone marrow osteoblastic cell cultures. Mechanical treated samples presented different Ra values and surface morphology. The hydrofluoric acid based solution (F solution) was effective in smoothing titanium surface and also in eliminating aluminum contamination resulting from the blasting process. Bone marrow cells seeded on the different titanium samples showed a similar pattern of behavior during cell attachment and spreading. Cells proliferated very well on all the titanium surfaces and cell growth was observed during two to three weeks. The samples treated with F solution presented higher ALP activity. Only the blasted samples treated with the acid solutions allowed seeded bone marrow cells to form a mineralized extracellular matrix. The best biological performance was found in the blasted samples treated with the hydrofluoric acid based solution, which could be related to the characteristic microtopography of these samples that presented a homogeneous and smooth roughness. Continuing this work a distribution of tensions at the bone-titanium interface will be simulated with the aid of software ANSYS.
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